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KMID : 0368420100530050374
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Journal of Plant Biology
2010 Volume.53 No. 5 p.374 ~ p.380
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F-box Protein Arabidillo-1 Promotes Lateral Root Development by Depressing the Functioning of GA3 in Arabidopsis
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Mu Changjun
Chen Ni
Li Xiaofeng
Jia Pengfei
Wang Zhaoyan
Liu Heng
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Abstract
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In Arabidopsis, Arabidillo-1 and Arabidillo-2 have great sequence homology to Dictyostelium and metazoan β?catenin/Armadillo, which are important to animal and Dictyostelium development. Arabidillo-1 and Arabidillo-2 promote lateral root formation redundantly in Arabidopsis. Here, we showed that gibberellins (GA3) has a greater inhibitory effect on lateral root growth from the null mutant arabidillo-1 than from the wild type, suggesting that the mechanism for Arabidillo-1-regulated modulation of lateral root proliferation is associated with GA3-metabolic or signaling pathways. Our yeast two-hybrid analysis demonstrated that Arabidillo-1 interacts with ASK2 and ASK11, and that ASK2 can bind with the F-box domain of Arabidillo-1. Therefore, Arabidillo-1 is involved in the ubiquitin/26S proteasome-mediated proteolytic pathway. Based on these results, we conclude that Arabidillo-1 can degrade some positive regulator of the GA3 signaling pathway through selective protein degradation of ubiquitin/26S. Moreover, that process is believed to be the mechanism for Arabidillo-1 promotion of lateral root development in Arabidopsis.
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KEYWORD
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Arabidillo-1, Armadillo, F-box, GA, Lateral roots
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